Authors:Placenta-derived decidua stromal cells (DSCs) are being investigated as an alternative to other sources of mesenchymal stromal cells (MSCs) for cellular therapy. DSCs are more effective in treating acute inflammatory diseases in human and this is our preclinical safety study of human DSCs in Sprague-Dawley rats and Balb/c mice. Human DSCs were cultured and expanded from fetal membranes obtained from placentas following cesarean section. In rats, 0.5 × 106 cells/kg were injected intravenously (n = 4) or intra-aortal (n = 4). In mice, DSCs were given intravenously at doses ranging from 4-40 × 106 cells/kg (total of n = 120 mice). In vivo tracking of human cells in mice was performed by using transduced DSC with luciferin gene, and in rats by using 18F-FDG PET. Clotting parameters were determined in vitro and in vivo. All intra-arterially DSC-treated rats had normal motility and behavior and histological examination was normal for liver, spleen kidneys and thigh muscles. Mice treated with DSCs showed no immediate or long-term side effects. None of the mice died or showed acute toxicity or adverse reactions 3 and 30 days after DSC infusion. Murine blood biochemistry profiles related to liver, kidney, heart, and inflammatory indices was not influenced by DSC infusion and complete blood counts were normal. In vivo tracking of infused DSCs detected a signal in the lungs for up to 4 days post infusion. Compared to bone marrow derived MSCs, the DSCs had better viability, smaller size, but stronger clotting in human blood and plasma. Both MSC- and DSC-induced coagulation and complement activation markers, thrombin-anti-thrombin complex (TAT) and C3a, and in vitro clotting parameters were decreased by heparin supplementation. In conclusion, DSCs are safe with almost no side effects even with doses 40 times higher than are used clinically, particularly when supplemented with low-dose heparin.

Title:Preclinical Toxicity Evaluation of Clinical Grade Placenta-Derived Decidua Stromal Cells

Journal:Front Immunol Year:2019; Volume:10

Authors:Zander T, Pan F, Schmidt H

Title:Which is the best-suited landmark to assess the thoracic orientation?

Journal:J Biomech Year:2019;

Authors:Kayaalp ME, Agres AN, Reichmann J, Bashkuev M, Duda GN, Becker R

Title:Validation of a Novel Device for the Knee Monitoring of Orthopaedic Patients

Year:2019; Volume:19Issue:(23)

Authors:Andrzejewska A, Catar R, Schoon J, Qazi TH, Sass FA, Jacobi D, Blankenstein A, Reinke S, Krüger D, Streitz M, Schlickeiser S, Richter S, Souidi N, Beez C, Kamhieh-Milz J, Krüger U, Zemojtel T, Jürchott K, Strunk D, Reinke P, Duda G, Moll G, Geissler S

Title:Multi-Parameter Analysis of Biobanked Human Bone Marrow Stromal Cells Shows Little Influence for Donor Age and Mild Comorbidities on Phenotypic and Functional Properties

Journal:Front Immunol Year:2019; Volume:10

Authors:Qazi TH, Mooney DJ, Duda GN, Geissler S

Title:Niche-mimicking interactions in peptide-functionalized 3D hydrogels amplify mesenchymal stromal cell paracrine effects

Journal:Biomaterials Year:2019;

Authors:Bashkuev M, Reitmaier S, Schmidt H

Title:Relationship between intervertebral disc and facet joint degeneration: A probabilistic finite element model study

Journal:J Biomech Year:2019;

Authors:Damm P, Reitmaier S, Hahn S, Waldheim V, Firouzabadi A, Schmidt H

Title:In vivo hip and lumbar spine implant loads during activities in forward bent postures

Journal:J Biomech Year:2019;

Authors:Borcherding K, Marx D, Gätjen L, Bormann N, Wildemann B, Specht U, Salz D, Thiel K, Grunwald I

Title:Burst Release of Antibiotics Combined with Long-Term Release of Silver Targeting Implant-Associated Infections: Design, Characterization and in vitro Evaluation of Novel Implant Hybrid Surface

Journal:Materials (Basel) Year:2019; Volume:12Issue:(23)

Authors:Pan F, Firouzabadi A, Zander T, Schmidt H

Title:Sex-dependent differences in lumbo-pelvic coordination for different lifting tasks: A study on asymptomatic adults

Journal:J Biomech Year:2019;

Authors:Pobloth AM, Mersiowsky MJ, Kliemt L, Schell H, Dienelt A, Pfitzner BM, Burgkart R, Detsch R, Wulsten D, Boccaccini AR,
Duda GN

Title:Bioactive coating of zirconia toughened alumina ceramic implants improves cancellous osseointegration

Journal:Sci Rep Year:2019; Volume:9Issue:(1)

Authors:Häske D, Schier L, Weerts JON, Groß B, Rittmann A, Grützner PA, Münzberg M, Kreinest M

Title:An explorative, biomechanical analysis of spine motion during out-of-hospital extrication procedures

Journal:Injury Year:2019;

Authors:Löffler J, Sass FA, Filter S, Rose A, Ellinghaus A, Duda GN, Dienelt A

Title:Compromised Bone Healing in Aged Rats Is Associated With Impaired M2 Macrophage Function

Journal:Front Immunol. Year:2019; Volume:Oct 18;10:2443. doi: 10.3389/fimmu.2019.02443. eCollection 2019.

Authors:Metz C, Duda GN, Checa S

Title:Towards multi-dynamic mechano-biological optimization of 3D-printed scaffolds to foster bone regeneration

Journal:Acta Biomater Year:2019;

Authors:Osterhoff G, Wulsten D, Babu S, Heyland M, Pari C

Title:Antegrade versus retrograde screw fixation of anterior column acetabular fractures: a biomechanical in vitro study

Journal:Eur J Trauma Emerg Surg Year:2019;

Authors:Differ C, Klatte-Schulz F, Bormann N, Minkwitz S, Knaus P, Wildemann B

Title:Is NO the Answer? The Nitric Oxide Pathway Can Support Bone Morphogenetic Protein 2 Mediated Signaling

Journal:Cells Year:2019; Volume:8Issue:(10)

Authors:Ort MJ, Geissler S, Rakow A, Schoon J

Title:The Allergic Bone Marrow? The Immuno-Capacity of the Human Bone Marrow in Context of Metal-Associated Hypersensitivity Reactions

Year:2019; Volume:10

Authors:Xiang G, Lippens E, Hafeez S, Duda GN, Geissler S, Qazi TH

Title:Oxidized alginate beads for tunable release of osteogenically potent mesenchymal stromal cells

Journal:Mater Sci Eng C Mater Biol Appl Year:2019; Volume:104

Authors:Kohn JC, Abdalrahman T, Sack KL, Reinhart-King CA, Franz T

Title:Cell focal adhesion clustering leads to decreased and homogenized basal strains

Journal:Int J Numer Method Biomed Eng Year:2019;

Authors:Pan F, Zander T, Reitmaier S, Bashkuev M, Schmidt H

Title:How reproducible do we stand and sit? Indications for a reliable sagittal spinal assessment

Journal:Clin Biomech Year:2019; Volume:70:Pages:123-130.

Authors:Schlundt C, Reinke S, Geissler S, Bucher CH, Giannini C, Märdian S, Dahne M, Kleber C, Samans B, Baron U, Duda GN, Volk HD, Schmidt-Bleek K

Title:Individual Effector/Regulator T Cell Ratios Impact Bone Regeneration

Journal:Front Immunol Year:2019; Volume:10