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    Comparison of acute and chronic tendon pathologies

    Klatte Schulz F. et al., Int J Mol Sci. 2018 Jan 30;19(2). pii: E404. doi: 10.3390/ijms19020404.

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    When to reimplant after joint infection?

    Winkler et at., Arch Orthop Trauma Surg 2019; 139(4):295-303.

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    Collagen scaffolds negatively impact bone healing ...

    Lang A. et al., Acta Biomaterialia 2019; 86:171-184.

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    Petersen et al.; Nature Communications; 2018

    Biomaterial-induced endochondral ossification to heal bone defects

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    Eckstein F. et al.; Osteoarthritis Cartilage; 2018

    Normal knee joints with contralateral joint space narrowing may serve as human model of early OA

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    Trepczynski et al.; J Neuroeng Rehabil.; 2018

    Antagonistic muscle co-contraction substantially increases in vivo knee contact forces

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    Klein et al.; Sci Rep.; 2018

    MALDI-IMS revealed that early molecular alterations occur largely in the region adjacent to the trauma

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    Lueckgen et al.; Biomaterials; 2018

    Hydrolytically-degradable click-crosslinked alginate hydrogels

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    Moll et al.; Trends in Molecular Medicine; 2019

    Intravascular Mesenchymal Stromal/Stem Cell Therapy Product Diversification: Time for New Clinical Guidelines

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Featured Publications

  • Moll G, Ankrum JA, Kamhieh-Milz J, Bieback K, Ringden O, Volk HD, Geissler S, Reinke P

    Intravascular Mesenchymal Stromal/Stem Cell Therapy Product Diversification: Time for New Clinical Guidelines


    Intravascular infusion is the most popular route for therapeutic multipotent mesenchymal stromal/stem cell (MSC) delivery in hundreds of clinical trials. Meta-analysis has demonstrated that bone marrow MSC infusion is safe. It is not clear if this also applies to diverse new cell products derived from other sources, such as adipose and perinatal tissues. Different MSC products display varying levels of highly procoagulant tissue factor (TF) and may adversely trigger the instant blood-mediated inflammatory reaction (IBMIR). Suitable strategies for assessing and controlling hemocompatibility and optimized cell delivery are crucial for the development of safer and more effective MSC therapies.

    Trends Mol Med 2019; 25(2):149-163.

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  • Lang A, Kirchner M, Stefanowski J, Durst M, Weber MC, Pfeiffenberger M, Damerau A, Hauser AE, Hoff P, Duda GN, Buttgereit F, Schmidt-Bleek K, Gaber T

    Collagen I-based scaffolds negatively impact fracture healing in a mouse-osteotomy-model although used routinely in research and clinical application


    Clinical application of bone morphogenetic protein 2 is only approved when applied on an absorbable bovine collagen I scaffold (ACS). Nevertheless, the influence of ACS alone on bone healing remains so far unknown. In vitro studies using ASC-H (Helistat) revealed a suppression of osteogenic differentiation and a reduction of cell vitality compared to ASC-L, another frequently used scaffold. In vivo, a significant delay in bone healing was observed when ACS-L was applied during femoral osteotomy. This negative influence of ACS-H and ACS-L on bone formation demonstraties a substantial need for more sophisticated delivery systems for local stimulation of bone healing.

    Acta Biomater 2019; 86:171-184.

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  • Eckstein F, Maschek S, Roemer FW, Duda GN, Sharma L, Wirth W

    Cartilage loss in radiographically normal knees depends on radiographic status of the contralateral knee - data from the Osteoarthritis Initiative


    OBJECTIVE: To test whether radiographically normal knees with contralateral radiographic knee osteoarthritis (OA), but without contralateral trauma history, display greater cartilage thickness loss than knees from subjects with bilaterally radiographically normal knees. CONCLUSIONS: Radiographically normal knees with contralateral JSN may serve as a human model of early OA, for testing disease modifying drugs in clinical trials designed to prevent cartilage loss before the onset of radiographic change. CLINICALTRIALS. GOV IDENTIFICATION: NCT00080171.

    Osteoarthritis Cartilage 2019; 27(2):273-277.

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  • Winkler T, Stuhlert MGW, Lieb E, Muller M, von Roth P, Preininger B, Trampuz A, Perka CF

    Outcome of short versus long interval in two-stage exchange for periprosthetic joint infection: a prospective cohort study


    For chronic periprosthetic joint infection, a 6-8 week interval is commonly used before re-implantation. We evaluated the influence of a short (< 4 weeks) and long (>/= 4 weeks) prosthesis-freeinterval on reinfection rate and functional outcome of hip and knee periprosthetic joint infection. Our study suggests that two-stage exchange with short interval has a similar outcome than with long interval, when highly active antibiotic therapy is used. Patient inconvenience and care costs due to immobilization were lower when strategies with a short interval were used.

    Arch Orthop Trauma Surg 2019; 139(4):295-303.

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  • Trepczynski A, Kutzner I, Schwachmeyer V, Heller MO, Pfitzner T, Duda GN

    Impact of antagonistic muscle co-contraction on in vivo knee contact forces


    BACKGROUND: The onset and progression of osteoarthritis, but also the wear and loosening of the components of an artificial joint, are commonly associated with mechanical overloading. Mechanical forces acting at the joints and understanding of the key factors that can alter them are critical to develop effective treatments for restoring joint function. While static anatomy is usually the clinical focus, less is known about the impact of dynamic factors, such as individual muscle recruitment, on joint contact forces. CONCLUSIONS: Treatment of diseased and failed joints should not only be restricted to anatomical reconstruction of static limb axes alignment. The dynamic activation of muscles, as a key modifier of lower limb biomechanics, should also be taken into account and thus also represents a promising target for restoring function, patient mobility, and preventing future joint failure. German Clinical Trials Register: ID: DRKS00000606

    J Neuroeng Rehabil 2018; 15(1):101.

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  • Petersen A, Princ A, Korus G, Ellinghaus A, Leemhuis H, Herrera A, Klaumunzer A, Schreivogel S, Woloszyk A, Schmidt-Bleek K, Geissler S, Heschel I, Duda GN

    A biomaterial with a channel-like pore architecture induces endochondral healing of bone defects


    Biomaterials developed to treat bone defects have classically focused on bone healing via direct, intramembranous ossification. In contrast, most bones in our body develop from a cartilage template via a second pathway called endochondral ossification. Here we report on a biomaterial with a channel-like pore architecture to control cell recruitment and tissue patterning in the early phase of healing. In consequence of extracellular matrix alignment, CD146+ progenitor cell accumulation and restrained vascularization, a highly organized endochondral ossification process is induced in rats. It demonstrates that a pure biomaterial approach can recapitulate a developmental bone growth process.

    Nat Commun 2018; 9(1):4430.

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  • Winkler T, Perka C, von Roth P, Agres AN, Plage H, Preininger B, Pumberger M, Geissler S, Hagai EL, Ofir R, Pinzur L, Eyal E, Stoltenburg-Didinger G, Meisel C, Consentius C, Streitz M, Reinke P, Duda GN, Volk HD

    Immunomodulatory placental-expanded, mesenchymal stromal cells improve muscle function following hip arthroplasty


    In this study we addressed surgical trauma-related muscle injuries resulting from Hip arthroplasty by local intraoperative application of allogeneic placenta-derived, mesenchymal-like adherent cells (PLX-PAD). This cell type was used because of its high regenerative and immunomodulatory potency and we could show that this allogeneic PLX-PAD therapy improved strength and volume of the injured skeletal muscle with a reasonable safety profile. Outcomes could be positively correlated with the modulation of early postoperative stress-related immunological reactions.

    J Cachexia Sarcopenia Muscle 2018; 9(5):880-897.

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  • Klein O, Strohschein K, Nebrich G, Fuchs M, Thiele H, Giavalisco P, Duda GN, Winkler T, Kobarg JH, Trede D, Geissler S

    Unraveling local tissue changes within severely injured skeletal muscles in response to MSC-based intervention using MALDI Imaging mass spectrometry


    Despite our increasing understanding of molecular and cellular mechanisms of MSCs regenerative function, little is known about the local molecular alterations and their spatial distribution within the tissue after autologous bone marrow MSC transplantation. Using MALDI imaging mass spectrometry and multivariate statistical strategies we revealed that very early molecular alterations in response to MSC transplantation occur largely in the region adjacent to the trauma and only to a small extent in the actual trauma region. We subsequently identified the proteins in the specific muscle regions.

    Sci Rep 2018; 8(1):12677.

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  • Lueckgen A, Garske DS, Ellinghaus A, Desai RM, Stafford AG, Mooney DJ, Duda GN, Cipitria A

    Hydrolytically-degradable click-crosslinked alginate hydrogels


    Degradable biomaterials aim to recapitulate the dynamic microenvironment that cells are naturally exposed to. By oxidizing the alginate polymer backbone, thereby rendering it susceptible to hydrolysis, and crosslinking it via norbornene-tetrazine click chemistry, we can control rheological, mechanical, and degradation properties of resulting hydrogels. The degradation behavior was verified by tracking mechanical and swelling behavior over time, showing that degradation could be decoupled from initial mechanical properties. Cells attached, spread and proliferated in 2D and retained a round morphology and stable number in 3D, while maintaining high viability in both contexts over 7 days. Finally, oxidized and unoxidized control materials were implanted subcutaneously into the backs of C57/Bl6 mice, and recovered after 8 weeks. Histological staining revealed morphological differences and fibrous tissue infiltration only in oxidized materials.

    Biomaterials 2018; 181:189-198.

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  • El Khassawna T, Serra A, Bucher CH, Petersen A, Schlundt C, Konnecke I, Malhan D, Wendler S, Schell H, Volk HD, Schmidt-Bleek K, Duda GN

    T Lymphocytes Influence the Mineralization Process of Bone


    Comparing bone and fracture healing in animals with and without mature T and B cells revealed their essential role during tissue mineralization processes and thus for the bone quality. Bone without mature T and B cells is stiffer compared to wild-type bone thus lacking the elasticity that helps to absorb forces to prevent fractures. In-depth analysis showed dysregulations in collagen deposition and osteoblast distribution when no mature T and B cells were present. This work presents, for the first time, a direct link between immune cells and matrix formation during bone healing after fracture. It illustrates specifically the role of T cells in the collagen organization process.

    Front Immunol 2017; 8:562.

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