Histology

Dr. med. vet. Anne-Marie Pobloth

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The equipment of our histology laboratories allows the making of plastic and paraffin preparations from soft and hard tissue.

Plastic embedded specimens

In this laboratory, plastic preparations can be made from non-decalcified hard tissue. Beside slices, grindings with or without metal implants can be prepared. By different histological stainings, several tissue types including mineralized tissue can be highlighted.

Histological stainings for light microscopy

Movat Pentachrome staining for overview and differentiation between mineralized and non-mineralized cartilage tissue

Trichrom Masson Goldner staining for overview and visualization of fibrous
tissue components

Alcian Blue staining for detection of acid mucosubstances

Safranin-Orange/von Kossa staining for visualization of mineralized tissue

Safranin-Orange/von Kossa staining for visualization of mineralized tissue

Paraffin embedded specimens

The equipment of the immunohistological laboratory allows the preparation of paraffin slices from decalcified hard and soft tissue. Several staining methods allow the characterization of different tissue and cell types.

Histological stainings for light microscopy

Hematoxylin and Eosin staining for overview

TRAP (tartrate-resistant acid phosphatase) histochemistry for detection of osteoclasts

Methylgreen / van Giesson staining for visualization of cartilage tissue

Immunohistochemical stainings

For detection of proteins by antigen-antibody reaction, the Avidin-Biotin method (peroxidase, alkaline phosphatase) is used. Beside several established antigen detection protocols, new protocols are established depending on the research Projects.

Immunohistochemistry for von Willebrand factor for detection of blood vessels (endothelial cells)

Immunhistochemischer Nachweis von alpha-Smooth-Muscle-Actin zur Darstellung von Blutgefäßen (Perizyten, glatte Muskelzellen) und Myofibroblasten

Immunohistochemistry for collagen type II

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