Local application

Prof. Dr. rer. nat. Britt Wildemann

Healing processes can be promoted by the application of active substances. A major field of research in orthopedics is the application of growth factors for healing stimulation and antibiotics for the prevention and treatment of infections.

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Mixing device for bone graft and drug
Experimental set-up for the in vitro investigation of the release kinetics of drugs from bone grafting material

Bone healing disorders and implant-associated infections are still a challenge in trauma and orthopedic surgery. Local application of active substances can promote endogenous healing processes, but also prevent or treat infections. At the same time, the necessary dosage and the systemic load is reduced, but also the availability on site is improved. Our group focuses on various possibilities of local application in various indications.

The developed biodegradable coating –based on the polymer poly(D,L-lactide)- for surgical material such as implants was extensively tested in vitro and in vivo for the application of antibiotics and bone healing stimulating factors. In addition to the optimization of the coating and the release kinetics, the effectiveness and biocompatibility was investigated.

Numerous bone substitutes are available for the treatment of bony defects. Ideally, the surgeon can enrich the appropriate material with an active substance to promote healing or fight bacteria. For this purpose, we have developed a perioperative approach which allows a fast and homogeneous mixture of substitute material with active substances and intensively analyzes the release kinetics of various antibiotics in vitro. Using a bone defect model we evaluated the effectivity of a bone graft (demineralized bone matrix, DBM) enriched with the osteoinductive factor BMP-2 by µCT and histology. We investigate the efficacy and cytotoxicity of newly discovered or modified antimicrobial substances in different in vitro approaches.

selected publications

Bormann N, Koliszak A, Kasper S, Schoen L, Hilpert K, Volkmer R, Kikhney J, Wildemann B.
A short artificial antimicrobial peptide shows potential to prevent or treat bone infections.
Sci Rep. 2017 May 4;7(1):1506.

Huber E, Pobloth A-M, Bormann N, Kolarczik N, Schmidt-Bleek K, Schell H, Schwabe P, Duda GN, Wildemann B.
DBM as a carrier for BMP-2: Burst release combined with long term binding and osteoinductive activity evaluated in vitro and in vivo.
Tissue Engineering Part A, 2017 epub

Back DA, Bormann N, Calafi A, Zech J, Garbe LA, Müller M, Willy C, Schmidmaier G, Wildemann B.
Testing of antibiotic releasing implant coatings to fight bacteria in combat-associated osteomyelitis - an in-vitro study.
Int Orthop. 2016 May;40(5):1039-47.

Nast S, Fassbender M, Bormann N, Beck S, Montali A, Lucke M, Schmidmaier G, Wildemann B.
In vivo quantification of gentamicin released from an implant coating.
J Biomater Appl. 2016 Jul;31(1):45-54.

Faßbender M, Minkwitz S, Strobel C, Schmidmaier G, Wildemann B.
Stimulation of bone healing by sustained bone morphogenetic protein 2 (BMP-2) delivery.
Int J Mol Sci. 2014 May 14;15(5):8539-52.

Fassbender M, Minkwitz S, Kronbach Z, Strobel C, Kadow-Romacker A, Schmidmaier G, Wildemann B.
Local gentamicin application does not interfere with bone healing in a rat model.
Bone. 2013 Aug;55(2):298-304

Pauly S, Back DA, Kaeppler K, Haas NP, Schmidmaier G, Wildemann B.
Influence of statins locally applied from orthopedic implants on osseous integration.
BMC Musculoskelet Disord. 2012 Oct 26;13:208.

Kadow-Romacker A, Greiner S, Schmidmaier G, Wildemann B.
Effect of β-tricalcium phosphate coated with zoledronic acid on human osteoblasts and human osteoclasts in vitro.
J Biomater Appl. 2013 Jan;27(5):577-85.

Strobel C, Bormann N, Kadow-Romacker A, Schmidmaier G, Wildemann B.
Sequential release kinetics of two (gentamicin and BMP-2) or three (gentamicin, IGF-I and BMP-2) substances from a one-component polymeric coating on implants.
J Control Release. 2011 Nov 30;156(1):37-45.

Wildemann B, Lange K, Strobel C, Fassbender M, Willie B, Schmidmaier G.
Local BMP-2 application can rescue the delayed osteotomy healing in a rat model.
Injury. 2011 Aug;42(8):746-52.