Personalized scaffold for bone defect regeneration
Additive manufacturing (AM) technologies could be the leverage for the generation of precise and personalized scaffold constructs enabling improved bone regeneration and remodeling outcomes. Our team strives to develop methodologies and generate knowledge that could lead to mechanistic understanding of scaffold-guided bone defect regeneration enabling precision in scaffold-assisted bone regenerative therapy.
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Featured Publications
Authors:Valainis D, Dondl P, Foehr P, Burgkart R, Kalkhof S, Duda GN, van Griensven M, Poh PSP
Title:Integrated additive design and manufacturing approach for the bioengineering of bone scaffolds for favorable mechanical and biological properties
Additive manufacturing (AM) presents the possibility of personalized bone scaffolds with unprecedented structural and functional designs. In contrast to the earlier conventional design concepts, e.g., raster-angle, a workflow was established to produce scaffolds with triply periodic minimal surface (TPMS) architecture. A core challenge is the realization of such structures using melt-extrusion based 3D printing. This study presents methods for generation of scaffold design files, finite element analysis of scaffold Young's moduli, AM of scaffolds with polycaprolactone (PCL), and a customized in vitro assay to evaluate cell migration. The reliability of FE analysis when using computer-aided designed models as input may be impeded by anomalies introduced into the scaffolds during 3D printing. Further investigation using micro-computed tomography reconstructions of printed scaffolds as an input for numerical simulation in comparison to experimentally obtained scaffold Young's moduli showed a moderate trend (R2 = 0.62). Interestingly, in a preliminary cell migration assay, adipose-derived mesenchymal stromal cells (AdMSC) migrated furthest on PCL scaffolds with Diamond, followed by Gyroid and Schwarz P architectures. A similar trend, but with an accelerated AdMSC migration rate, was observed for PCL scaffolds surface coated with calcium-phosphate-based apatite. We elaborate on the importance of start-to-finish integration of all steps of AM, i.e. design, engineering and manufacturing. Using such a workflow, specific biological and mechanical functionality, e.g., improved regeneration via enhanced cell migration and higher structural integrity, may be realized for scaffolds intended as temporary guiding structures for endogenous tissue regeneration.
Journal:Biomed Mater Year:2019;
Authors:Poh PSP., Valainis D, Bhattacharya K, van Griensven M, Dondl P
Title:Optimization of Bone Scaffold Porosity Distributions
Additive manufacturing (AM) is a rapidly emerging technology that has the potential to produce personalized scaffolds for tissue engineering applications with unprecedented control of structural and functional design. Particularly for bone defect regeneration, the complex coupling of biological mechanisms to the scaffolds' properties has led to a predominantly trial-and-error approach. To mitigate this, shape or topology optimization can be a useful tool to design a scaffold architecture that matches the desired design targets, albeit at high computational cost. Here, we consider an efficient macroscopic optimization routine based on a simple one-dimensional time-dependent model for bone regeneration in the presence of a bioresorbable polymer scaffold. The result of the optimization procedure is a scaffold porosity distribution which maximizes the stiffness of the scaffold and regenerated bone system over the entire regeneration time, so that the propensity for mechanical failure is minimized.
Journal:Sci Rep Year:2019; Volume:9Issue:(1):Pages:9170.
Publications
Results 1 to 10 of total 11
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- Authors:Amann E, Amirall A , Franco AR, Poh PSP, Dueñas FJS, Estévez GF, Leonor IB, Reis RL, van Griensven M, Balmayor ER
Journal:Adv Healthc Mater Year:2021;
Title:A Graded, Porous Composite of Natural Biopolymers and Octacalcium Phosphate Guides Osteochondral Differentiation of Stem Cells - Authors:Munkhbaatar E, Dietzen M, Agrawal D, Anton M, Jesinghaus M, Boxberg M, Pfarr N, Bidola P, Uhrig S, Höckendorf U, Meinhardt AL, Wahida A, Heid I, Braren R, Mishra R, Warth A, Muley TH, Poh PSP, ……
Journal:Nat Commun Year:2020; Volume:11Issue:(1):Pages:4527.
Title:MCL-1 gains occur with high frequency in lung adenocarcinoma and can be targeted therapeutically - Authors:Valainis D, Dondl P, Foehr P, Burgkart R, Kalkhof S, Duda GN, van Griensven M, Poh PSP
Journal:Biomed Mater Year:2019;
Title:Integrated additive design and manufacturing approach for the bioengineering of bone scaffolds for favorable mechanical and biological properties - Authors:Poh PSP., Valainis D, Bhattacharya K, van Griensven M, Dondl P
Journal:Sci Rep Year:2019; Volume:9Issue:(1):Pages:9170.
Title:Optimization of Bone Scaffold Porosity Distributions - Authors:Hammerl A, Diaz Cano CE,3, De-Juan-Pardo EM, van Griensven M, Poh PSP
Journal:Int J Mol Sci Year:2019; Volume:20Issue:(5):Pages:pii: E1068. doi: 10.3390/ijms20051068.
Title:A Growth Factor-Free Co-Culture System of Osteoblasts and Peripheral Blood Mononuclear Cells for the Evaluation of the Osteogenesis Potential of Melt-Electrowritten Polycaprolactone Scaffold - Authors:Wichlas F, Tsitsilonis S, Kopf S, Krapohl BD, Manegold S
Journal:GMS Interdiscip Plast Reconstr Surg DGPW Year:2017; Volume:6
Title:Fracture heuristics: surgical decision for approaches to distal radius fractures. A surgeon's perspective. - Authors:Tsitsilonis S, Machó D, Manegold S, Krapohl BD, Wichlas F
Journal:GMS Interdiscip Plast Reconstr Surg DGPW Year:2016; Volume:5
Title:Fracture severity of distal radius fractures treated with locking plating correlates with limitations in ulnar abduction and inferior health-related quality of life. - Authors:Rohlmann A, Pohl D, Bender A, Graichen F, Dymke J, Schmidt H, Bergmann G
Journal:PLoS One Year:2014; Volume:9Issue:(5):Pages:e98510.
Title:Activities of everyday life with high spinal loads - Authors:Garcia P, Histing T, Holstein JH, Klein M, Laschke MW, Matthys R, Ignatius A, Wildemann B, Lienau J, Peters A, Willie B, Duda G, Claes L, Pohlemann T, Menger MD
Journal:Eur Cell Mater Year:2013; Volume:26:Pages:1-12; discussion 12-4.
Title:Rodent animal models of delayed bone healing and non-union formation: a comprehensive review - Authors:Histing T, Garcia P, Holstein JH, Klein M, Matthys R, Nuetzi R, Steck R, Laschke MW, Wehner T, Bindl R, Recknagel S, Stuermer EK, Vollmar B, Wildemann B, Lienau J, Willie B, Peters A, Ignatius A, Pohlemann T, Claes L, Menger MD
Journal:Bone Year:2011; Volume:49Issue:(4):Pages:591-9.
Title:Small animal bone healing models: standards, tips, and pitfalls results of a consensus meeting
Results 1 to 10 of total 11
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