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The design of the bioreactor was motivated by the idea to mimic the situation during bone healing in an established sheep osteotomy model by an in vitro system. The system allows the application of various compression and load patterns to 3D constructs mimicking the fracture hematoma or soft callus. This happens in a closed environment under dynamic cultivation (medium circulation) and control of cell culture parameters like oxygen and pH. As a special feature, the mechanical properties of cell seeded constructs can be measured in situ and monitored online during cultivation. This allows analyzing the impact of cellular behaviour on the macroscopic mechanical properties. By implementing the gas exchange into the medium reservoir and by utilizing gas mixing devices, specific environments (e.g. hypoxia) can be realized.
Additionally the individual bioreactors units can be run in any temperature controlled environment and outside of CO2 incubators. Patent pending (Application Nr.: EP20090175335).