Tendon and Bone Regeneration

We are an interdisciplinary research group working tightly together with the clinical colleagues. This cooperation gives us the possibility to identify the problems in orthopedic and trauma surgery and to work on new strategies to solve them.

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Histology

The equipment of our histology laboratories allows the making of plastic and paraffin preparations from soft and hard tissue.

Plastic Embeded Histology

In this laboratory, plastic preparations can be made from non-decalcified hard tissue. Beside slices, grindings with or without metal implants can be prepared. By different histological stainings, several tissue types including mineralized tissue can be highlighted.

Histological stainign for light microscopy

Movat Pentachrome staining for overview and differentiation between mineralized and non-mineralized cartilage tissue
Trichrom staining in accordance with Masson-Goldner as overview staining and for the visualisation of connective tissue
Alcian blue staining for the detection of acid mucus substances
Safranin orange / von Kossa staining for the detection of mineralised tissue
Alzarin red staining for the detection of mineralised tissue

Parafin Histology

The equipment of the immunohistological laboratory allows the preparation of paraffin slices from decalcified hard and soft tissue. Several staining methods allow the characterization of different tissue and cell types.

Histological stainings for light microscopy

Hematoxylin and Eosin staining for overview
TRAP (Tartrat resistant acid phosphatase) Histochemistry for the detection of osteoclasts
Methyl green / van Gieson staining for the detection of cartilage

Hematoxylin and Eosin staining for overview

Immunohistochemical Staining

For detection of proteins by antigen-antibody reaction, the Avidin-Biotin method (peroxidase, alkaline phosphatase) is used. Beside several established antigen detection protocols, new protocols are established depending on the research Projects.

Immunohistochemistry detection of alpha-Smooth-Muscle-Actin for the visualisation of blood vesseis (Pericytes, smooth muscle cells) und myofibroblasts
Immunohistochemistry for von Willebrand factor for detection of blood vessels (endothelial cells)
Immunohistochemistry detection of collagen type II